Agrobacterium tumefaciens Deploys a Superfamily of Type VI Secretion DNase Effectors as Weapons for Interbacterial Competition In Planta

The type VI secretion system (T6SS) is a widespread molecular weapon deployed by many Proteobacteria to target effectors/toxins into both eukaryotic and prokaryotic cells. We report that Agrobacterium tumefaciens, a soil bacterium that triggers tumorigenesis in plants, produces a family of type VI DNase effectors (Tde) that are distinct from previously known polymorphic toxins and nucleases. Tde exhibits an antibacterial DNase activity that relies on a conserved HxxD motif and can be counteracted by a cognate immunity protein, Tdi. In vitro, A. tumefaciens T6SS could kill Escherichia coli but triggered a lethal counterattack by Pseudomonas aeruginosa upon injection of the Tde toxins. However, in an in planta coinfection assay, A. tumefaciens used Tde effectors to attack both siblings cells and P. aeruginosa to ultimately gain a competitive advantage. Such acquired T6SS-dependent fitness in vivo and conservation of Tde-Tdi couples in bacteria highlights a widespread antibacterial weapon beneficial for niche colonization

Solving the Darwin problem in the first post-Newtonian approximation of general relativity

We analytically calculate the equilibrium sequence of the corotating binary stars of incompressible fluid in the first post-Newtonian(PN) approximation of general relativity. By calculating the total energy and total angular momentum of the system as a function of the orbital separation, we investigate the innermost stable circular orbit for corotating binary(we call it ISCCO). It is found that by the first PN effect, the orbital separation of the binary at the ISCCO becomes small with increase of the compactness of each star, and as a result, the orbital angular velocity at the ISCCO increases. These behaviors agree with previous numerical works.Comment: 33 pages, revtex, 4 figures(eps), accepted for publication in Phys. Rev.

Dynamical evolution and leading order gravitational wave emission of Riemann-S binaries

An approximate strategy for studying the evolution of binary systems of extended objects is introduced. The stars are assumed to be polytropic ellipsoids. The surfaces of constant density maintain their ellipsoidal shape during the time evolution. The equations of hydrodynamics then reduce to a system of ordinary differential equations for the internal velocities, the principal axes of the stars and the orbital parameters. The equations of motion are given within Lagrangian and Hamiltonian formalism. The special case when both stars are axially symmetric fluid configurations is considered. Leading order gravitational radiation reaction is incorporated, where the quasi-static approximation is applied to the internal degrees of freedom of the stars. The influence of the stellar parameters, in particular the influence of the polytropic index $n$, on the leading order gravitational waveforms is studied.Comment: 31 pages, 7 figures, typos correcte

Innermost Stable Circular Orbit of Inspiraling Neutron-Star Binaries: Tidal Effects, Post-Newtonian Effects and the Neutron-Star Equation of State

We study how the neutron-star equation of state affects the onset of the dynamical instability in the equations of motion for inspiraling neutron-star binaries near coalescence. A combination of relativistic effects and Newtonian tidal effects cause the stars to begin their final, rapid, and dynamically-unstable plunge to merger when the stars are still well separated and the orbital frequency is $\approx$ 500 cycles/sec (i.e. the gravitational wave frequency is approximately 1000 Hz). The orbital frequency at which the dynamical instability occurs (i.e. the orbital frequency at the innermost stable circular orbit) shows modest sensitivity to the neutron-star equation of state (particularly the mass-radius ratio, $M/R_o$, of the stars). This suggests that information about the equation of state of nuclear matter is encoded in the gravitational waves emitted just prior to the merger.Comment: RevTeX, to appear in PRD, 8 pages, 4 figures include

General-relativistic coupling between orbital motion and internal degrees of freedom for inspiraling binary neutron stars

We analyze the coupling between the internal degrees of freedom of neutron stars in a close binary, and the stars' orbital motion. Our analysis is based on the method of matched asymptotic expansions and is valid to all orders in the strength of internal gravity in each star, but is perturbative in the ``tidal expansion parameter'' (stellar radius)/(orbital separation). At first order in the tidal expansion parameter, we show that the internal structure of each star is unaffected by its companion, in agreement with post-1-Newtonian results of Wiseman (gr-qc/9704018). We also show that relativistic interactions that scale as higher powers of the tidal expansion parameter produce qualitatively similar effects to their Newtonian counterparts: there are corrections to the Newtonian tidal distortion of each star, both of which occur at third order in the tidal expansion parameter, and there are corrections to the Newtonian decrease in central density of each star (Newtonian ``tidal stabilization''), both of which are sixth order in the tidal expansion parameter. There are additional interactions with no Newtonian analogs, but these do not change the central density of each star up to sixth order in the tidal expansion parameter. These results, in combination with previous analyses of Newtonian tidal interactions, indicate that (i) there are no large general-relativistic crushing forces that could cause the stars to collapse to black holes prior to the dynamical orbital instability, and (ii) the conventional wisdom with respect to coalescing binary neutron stars as sources of gravitational-wave bursts is correct: namely, the finite-stellar-size corrections to the gravitational waveform will be unimportant for the purpose of detecting the coalescences.Comment: 22 pages, 2 figures. Replaced 13 July: proof corrected, result unchange

Black Hole Production by Cosmic Rays

Ultra-high energy cosmic rays create black holes in scenarios with extra dimensions and TeV-scale gravity. In particular, cosmic neutrinos will produce black holes deep in the atmosphere, initiating quasi-horizontal showers far above the standard model rate. At the Auger Observatory, hundreds of black hole events may be observed, providing evidence for extra dimensions and the first opportunity for experimental study of microscopic black holes. If no black holes are found, the fundamental Planck scale must be above 2 TeV for any number of extra dimensions.Comment: 4 pages, 4 figures, PRL versio

Quasi-Local Energy Flux of Spacetime Perturbation

A general expression for quasi-local energy flux for spacetime perturbation is derived from covariant Hamiltonian formulation using functional differentiability and symplectic structure invariance, which is independent of the choice of the canonical variables and the possible boundary terms one initially puts into the Lagrangian in the diffeomorphism invariant theories. The energy flux expression depends on a displacement vector field and the 2-surface under consideration. We apply and test the expression in Vaidya spacetime. At null infinity the expression leads to the Bondi type energy flux obtained by Lindquist, Schwartz and Misner. On dynamical horizons with a particular choice of the displacement vector, it gives the area balance law obtained by Ashtekar and Krishnan.Comment: 8 pages, added appendix, version to appear in Phys. Rev.